Inhibitory effects of Calocybe indica macrofungi on experimental benign prostatic hyperplasia in rats.

Objectives: This study was designed to investigate the protective effects of Calocybe indica extract on testosterone-induced benign prostatic hyperplasia in rats. Materials and Methods: In this study, 60 adult Sprague Dawley rats were randomly divided into six equal groups, one group served as the normal control, five of the groups were administered subcutaneous testosterone propionate for 28 days to induce benign prostatic hyperplasia, three of the five groups were simultaneously administered three graded doses of C. indica extract while one group was administered finasteride as the standard drug and the other left as untreated BPH model group given testosterone propionate only. BPH in the prostate gland was detected through gross appearance, prostate weight, and biochemical and histopathological analyses. Results: Increased prostate weight, serum prostate-specific antigen (PSA), and epithelial thickness were observed in the untreated testosterone-induced BPH model. Administration of finasteride and C. indica extract led to a reduction in prostate weight, prostatic index, serum PSA, serum levels of testosterone, and prostatic epithelial thickness, and increased luminal diameter. Conclusion: Administration of C. indica extract suppressed the pathophysiological effects of benign prostatic hyperplasia in rats. Thus, C. indica mushroom is a potential pharmacological candidate for the management of BPH in man or dogs.

Some Biochemical Perturbations May Modify the Understanding of Trypanotolerance in the West African Dwarf Sheep Infected With Trypanosoma brucei brucei and Trypanosoma congolense.

Trypanosomes are single-celled protozoa that cause severe diseases in both humans and livestock in sub-Saharan African countries. The disease in the West African Dwarf (WAD) sheep is often neglected due to the issue of trypanotolerance. The current study is aimed to evaluate some biochemical changes in this breed that may modify the understanding of trypanotolerance. Fifteen WAD sheep were assigned into 3 groups (A, B, and C). Baseline (day 0) values of the parameters assayed were obtained before groups A and B were infected with Trypanosoma brucei brucei and Trypanosoma congolense, respectively, by intraperitoneal inoculation with 106 trypanosomes per animal. Standard procedures using Quimica Clinica Applicada (Spain) and Randox (UK) test kits were used to evaluate serum levels of AST, ALT, ALP, total protein, albumin, total cholesterol, urea, and creatinine on days 0, 14, 28, 42, 56, and 70 post infection. The infections caused sustained pyrexia, hypoproteinaemia, hypocholesterolaemia, weight loss, hepatitis, and mortalities although parasitaemia was greatly controlled especially in the T congolense infected rams. The findings suggest that the WAD rams are not just passive reservoirs of trypanosomes for human and animal infections, but experience active host-parasite interactions with huge price for resilience, biochemically.